Article
Detection of basepair substitution mutation at a frequency of 1 x 10(-7) by combining two genotypic selection methods, MutEx enrichment and allele-specific competitive blocker PCR.
Environmental and molecular mutagenesis - 1 Jan 1998
Parsons B L, Heflich R H
Abstract excerpt
The detection of rare mutations has many important applications, including risk assessment of drugs and chemicals, measuring environmental exposures to genotoxins, and cancer cell detection. A sensitive genotypic selection method has been developed that combines two different mutant allele select...
Topics
- Adenosine Triphosphatases
- Animals
- Bacterial Proteins
- Carcinogens
- DNA Mutational Analysis
- DNA-Binding Proteins
- DNA-Directed DNA Polymerase
- Escherichia coli
- Escherichia coli Proteins
- Genes, ras
- Genotype
- Mice
- MutS DNA Mismatch-Binding Protein
- Nucleic Acid Heteroduplexes
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Thermus