Article
High-throughput screening approach identifies substrate-selective Hsp104 variants that counter amyloid seeding with diminished off-target effects.
Molecular cell - 21 May 2026
Ryan Jeremy J, Miller Karlie R, Puri Anuradhika, Sprunger Macy L, Bochantine Madalyn M, Lopez April, Chen Sheng, Bao Aaron, Ling Cendi, Xu Andy, Bell Braxton, Sohn Brian S, Li Leo, Chang Cynthia, Mohammed Kalid, Staller Max V, Jackrel Meredith E
Abstract excerpt
Hsp104, a yeast protein-remodeling factor, can disaggregate misfolded proteins implicated in neurodegeneration. Although many potentiated Hsp104 variants have been generated, suboptimal properties have limited their application in mammalian systems. Here, we present the development of a high-throughput screening approach for identifying enhanced Hsp104 variants. To screen a large library of variants in parallel...
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