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Article

A naturally monomeric infrared fluorescent protein for protein labeling in vivo.

Nature methods - 1 Aug 2015

Yu Dan, Baird Michelle A, Allen John R, Howe Elizabeth S, Klassen Matthew P, Reade Anna, Makhijani Kalpana, Song Yuanquan, Liu Songmei, Murthy Zehra, Zhang Shao-Qing, Weiner Orion D, Kornberg Thomas B, Jan Yuh-Nung, Davidson Michael W, Shu Xiaokun

Abstract excerpt

Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its homologs in protein labeling. Drawing on structural analysis of the dimer interface, we identified a bacteriophytochrome in the sequence database that is monomeric in truncated form and engineered it into a naturally monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live cells, Drosophila and zebrafish. It...

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Identifiers

PMID: 26098020
DOI: 10.1038/nmeth.3447
PMCID: PMC4521985

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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