Using CRISPR barcoding as a molecular clock to capture dynamic processes at single-cell resolution.

doi:10.1101/gr.280915.125

Article

Using CRISPR barcoding as a molecular clock to capture dynamic processes at single-cell resolution.

Genome research - 6 May 2026

Andres-Lopez Yolanda, Santambrogio Alice, Kafetzopoulos Ioannis, Todd Christopher D, El Khouri-Gonzalez Carla, Gonzalez-Alvarez J-Elliot, Alda-Catalinas Celia, Clark Stephen J, Reik Wolf, Hernando-Herraez Irene

Abstract excerpt

Biological processes are inherently dynamic, yet current methods for capturing temporal changes remain limited. Here, we present scDynaBar, a novel approach that combines CRISPR-Cas9 dynamic barcoding with single-cell sequencing. In this system, genetic barcodes gradually accumulate mutations over time; these barcodes are sequenced alongside the transcriptome of individual cells. We propose that the divergence of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Using CRISPR barcoding as a molecular clock to capture dynamic processes at single-cell resolution.

Abstract excerpt

Topics

Related research