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Article

Imaging of individual transcripts by amplification-based single-molecule fluorescence in situ hybridization.

New biotechnology - 25 Mar 2021

Lin Chen, Jiang Meng, Liu Ling, Chen Xiaoyuan, Zhao Yuancun, Chen Lu, Hong Yujuan, Wang Xin, Hong Chengye, Yao Xihu, Ke Rongqin

Abstract excerpt

An amplification-based single-molecule fluorescence in situ hybridization (asmFISH) assay is introduced that exploits improved probe design for highly specific imaging of individual transcripts in fixed cells and tissues. In this method, a pair of DNA ligation probes are ligated on RNA templates upon specific hybridization, followed by probe circularization based on enzymatic DNA ligation and rolling circle...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33301924
DOI: 10.1016/j.nbt.2020.12.001

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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