BNANC clamp PCR for simple and sensitive detection of KRAS and EGFR mutations.

Abstract excerpt

2',4'-BNANC (BNANC)-modified oligonucleotides, which have excellent binding affinity to complementary RNA and DNA strands and high nuclease resistance, were successfully used to develop a new clamp polymerase chain reaction (PCR) method for detecting trace amounts of mutant DNA in the presence of excess wild-type DNA. The BNANC clamp oligonucleotides (BNANC clamps) showed excellent clamping ability compared with...

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• ErbB Receptors
• EGFR gene
• KRAS gene
• Mutation
• Oligonucleotides