Article
Wild‑type blocking pcr coupled with internal competitive amplified fragment improved the detection of rare mutation of KRAS.
Molecular medicine reports - 1 Sept 2017
Peng Jia, Wei Kun, Zhao Xiang, Yang Ke, Wang Huan, Zhang Yang, Guo Mei, He Jing, Wu Haiyan, Li Yongchuan, Zhao Na, Huang Qing, Fu Weiling
Abstract excerpt
Mutant KRAS proto‑oncogene GTPase (KRAS) serves an important role in predicting the development, diagnosis, treatment and efficacy of targeted drug therapies for colorectal cancer. To improve the detection efficacy of trace amount of mutant KRAS, the locked nucleic acid‑based method was modified in the present study. Internal competitive amplification fragments were used to improve the inhibition of wild‑type...
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