Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Biosensor-assisted titratable CRISPRi high-throughput (BATCH) screening for over-production phenotypes.

Metabolic engineering - 1 Jan 2023

Wang Jian, Li Chenyi, Jiang Tian, Yan Yajun

Abstract excerpt

With rapid advances in the development of metabolic pathways and synthetic biology toolkits, a persisting challenge in microbial bioproduction is how to optimally rewire metabolic fluxes and accelerate the concomitant high-throughput phenotype screening. Here we developed a biosensor-assisted titratable CRISPRi high-throughput (BATCH) screening approach that combines a titratable mismatch CRISPR interference and...

Topics

Join the communities discussing this publication.

Coumaric Acids
CRISPR-Cas Systems
Escherichia coli
High-Throughput Screening Assays
Metabolic Engineering

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Open in PubMed Open full text

Identifiers

PMID: 36375746
DOI: 10.1016/j.ymben.2022.11.004
PMCID: PMC9845192

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Biosensor-assisted titratable CRISPRi high-throughput (BATCH) screening for over-production phenotypes.PMID 36375746Read ↗

A broad-host-range CRISPRi toolkit for silencing gene expression in <i>Burkholderia</i>Semantic proximity Read ↗

Pooled CRISPR screening enables genotype-phenotype association in industrial microorganisms.Semantic proximity Read ↗

Selection of chromosomal DNA libraries using a multiplex CRISPR system.Semantic proximity Read ↗

CRISPR-Cas tools for simultaneous transcription & translation control in bacteriaSemantic proximity Read ↗

Cell-Free Transcription-Coupled CRISPR/Cas12a Assay for Prototyping Cyanobacterial Promoters.Semantic proximity Read ↗

Multiplexed in-situ mutagenesis driven by a dCas12a-based dual-function base editor.Semantic proximity Read ↗

In vivo continuous evolution via phenotypic sorting to alleviate metabolic bottlenecks in β-alanine production.Semantic proximity Read ↗

mpCRISTAR: Multiple Plasmid Approach for CRISPR/Cas9 and TAR-Mediated Multiplexed Refactoring of Natural Product Biosynthetic Gene Clusters.Semantic proximity Read ↗

Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profilingSemantic proximity Read ↗

Identification of functional sgRNA mutants lacking canonical secondary structure using high-throughput FACS screening.Semantic proximity Read ↗

Combinatorial pathway engineering using type I-E CRISPR interference.Semantic proximity Read ↗

A simplified strategy for titrating gene expression reveals new relationships between genotype, environment, and bacterial growth.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.