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Thermostability Enhancement of Streptomyces mobaraensis Transglutaminase by Folding Free Energy-Guided Helix-Corner Motif Engineering.

Journal of agricultural and food chemistry - 25 Mar 2026

Tan Yameng, Yang Penghui, Qiu Wenxuan, Xue Hongjian, Du Guocheng, Zhou Jingwen, Liu Song

Abstract excerpt

The industrial application of Streptomyces mobaraensis transglutaminase (smTG) is limited by its poor thermostability. To enhance its stability, we engineered a thermostable mutant, FRAPD-TGm2A1, using helix-corner design. Following the introduction of the known stabilizing mutation Y34W, Cartesian_ddG was used to virtually screen 56 helix-corner residue sites, from which 20 top-ranking mutations with reduced...

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PMID: 41841186
DOI: 10.1021/acs.jafc.6c01252

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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