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Article

Enhancing the thermostability of alpha-glucosidase from Thermoanaerobacter tengcongensis MB4 by single proline substitution.

Journal of bioscience and bioengineering - 1 Jul 2010

Zhou Cheng, Xue Yanfen, Ma Yanhe

Abstract excerpt

Thermostability can be increased by introducing prolines at suitable sites in target proteins. In this study, we compared five thermostable alpha-glucosidases and the moderate thermostable alpha-glucosidase (TtGluA) from Thermoanaerobacter tengcongensis MB4. Based on the amino acid sequence alignment, four sites (Leu152, Asn208, Lys285, and Thr430) of TtGluA were chosen for proline substitution to improve its...

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PMID: 20541109
DOI: 10.1016/j.jbiosc.2009.12.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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