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Article

Improvement of the activity and thermostability of microbial transglutaminase by multiple-site mutagenesis.

Bioscience, biotechnology, and biochemistry - 1 Jan 2018

Mu Dongdong, Lu Jiaojiao, Shu Chang, Li Haowen, Li Xingjiang, Cai Jing, Luo Shuizhong, Yang Peizhou, Jiang Shaotong, Zheng Zhi

Abstract excerpt

Microbial transglutaminase (MTG) is an enzyme widely used in the food industry. Mutiple-site mutagenesis of Streptomyces mobaraensis transglutaminase was performed in Escherichia coli. According to enzymatic assay and thermostability study, among three penta-site MTG mutants (DM01-03), DM01 exhibited the highest enzymatic activity of 55.7 ± 1.4 U/mg and longest half-life at 50 °C (418.2 min) and 60 °C (24.8 min).

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Identifiers

PMID: 29198166
DOI: 10.1080/09168451.2017.1403881

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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