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Article

Screening for improved activity of a transglutaminase from Streptomyces mobaraensis created by a novel rational mutagenesis and random mutagenesis.

Applied microbiology and biotechnology - 1 Aug 2010

Yokoyama Keiichi, Utsumi Hiroe, Nakamura Takefumi, Ogaya Daisuke, Shimba Nobuhisa, Suzuki Eiichiro, Taguchi Seiichi

Abstract excerpt

Microbial transglutaminase (MTG) has been used extensively in academic research and the food industries through its cross-linking or posttranslational modification of proteins. Two enzyme engineering approaches were applied to improve MTG activity. One is a novel method of rational mutagenesis, called water-accessible surface hot-space region-oriented mutagenesis (WASH-ROM). One hundred and fifty-one point...

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Identifiers

PMID: 20521043
DOI: 10.1007/s00253-010-2656-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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