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Construction, expression, purification, characterization, and structural analysis of microbial transglutaminase variants.

Biotechnology and applied biochemistry - 1 Dec 2022

Song Xiaoping, Sheng Hefang, Zhou Yueqiao, Yu Yin, He Yingjiao, Wang Zihan

Abstract excerpt

Microbial transglutaminase (MTG, EC 2.3.2.13) derived from Streptomyces mobaraensis is widely used in the food and pharmaceutical industry because of its ability to synthesize isopeptide bonds between the proteinogenic side chains of glutamine and lysine. The half-life (t1/2 ) of the activated wild-type enzyme at 60°C is 2 min. To improve the activity and thermostability of MTG for higher temperature application,...

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PMID: 34894362
DOI: 10.1002/bab.2298

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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