Article
Development of Multiplex Drop-Off Digital PCR Assays for Hotspot Mutation Detection of KRAS, NRAS, BRAF, and PIK3CA in the Plasma of Colorectal Cancer Patients.
The Journal of molecular diagnostics : JMD - 1 Jun 2023
Yu Qian, Jiang Huiqin, Su Xi, Jiang Zhengxin, Liang Xue, Zhang Chunyan, Shang Wu, Zhang Yiliang, Chen Hao, Yang Zhijie, Shen Minna, Huang Fei, Chen Xinning, Yang Yihui, Pan Baishen, Wang Beili, Lu Daru, Guo Wei
Abstract excerpt
The detection of mutations in KRAS, NRAS, BRAF, and PIK3CA has become essential in managing the treatment of metastatic colorectal cancer (CRC) with the approval of new targeted therapies. We developed novel multiplex drop-off digital PCR (MDO-dPCR) assays by combining amplitude-/ratio-based multiplexing with drop-off/double drop-off strategies that allow for the detection of at least the 69 most frequent hotspot...
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