From random to chemoselective cross-linking: Reorientation and enhancement of immobilized cross-linked enzyme aggregates of cyclodextrin glucanotransferase from Shouchella lehensis G1 via targeted domain modification.

Abstract excerpt

Uncontrolled, random cross-linking near the enzyme's active site may impede mass transfer and overall activity, which is one of the challenges in cross-linked enzyme aggregates (CLEAs) immobilization. Likewise, a thorough understanding of intermolecular interactions between cross-linkers and enzymes is crucial to yield robust and oriented CLEAs. Therefore, this study aims to chemoselectively cross-link...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Cross-Linking Reagents
• Share to Glucosyltransferases