Article
Analysis of the Effect of N555 Mutations on the Product Specificity of Dextransucrase Using Caffeic Acid Phenethyl Ester as an Acceptor Substrate.
Journal of agricultural and food chemistry - 26 May 2021
Yu Xiao-Qin, Yang Jing-Wen, Ding Xiao-Jie, Liu Lan-Hua, Hu Xue-Qin, Zhang Hong-Bin
Abstract excerpt
Bioglycosylation is an efficient strategy to improve biological activities and physicochemical properties of natural compounds to develop structural modifications of drugs. In this study, an N555 residue was identified as a candidate for site-directed mutagenesis through sequence alignment with GTF180ΔN. Caffeic acid phenethyl ester (CAPE) was used as an acceptor substrate. Two generated mutants, N555Q and N555E,...
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