Engineering of β-glucosidase for rare ginsenoside biosynthesis via distal conformational fine-tuning and sequential degradation control.

Abstract excerpt

The structural complexity and regiospecificity of ginsenosides pose significant challenges for precise biocatalysis, necessitating enzymes with finely tuned conformational control. Here, we report the high-level biomanufacturing of the rare ginsenoside F2 via computational redesign of β-glucosidase BglPp from Paenibacillus phyllosphaerae. By integrating evolutionary fitness landscapes with thermodynamic stability...

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