Article
Glucansucrase Gtf180-ΔN of Lactobacillus reuteri 180: enzyme and reaction engineering for improved glycosylation of non-carbohydrate molecules.
Applied microbiology and biotechnology - 1 Sept 2016
Devlamynck Tim, Te Poele Evelien M, Meng Xiangfeng, van Leeuwen Sander S, Dijkhuizen Lubbert
Abstract excerpt
Glucansucrases have a broad acceptor substrate specificity and receive increased attention as biocatalysts for the glycosylation of small non-carbohydrate molecules using sucrose as donor substrate. However, the main glucansucrase-catalyzed reaction results in synthesis of α-glucan polysaccharides from sucrose, and this strongly impedes the efficient glycosylation of non-carbohydrate molecules and complicates...
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