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Article

Phenotypic CRISPR screens identify NLRX1 as an essential activator of the human mitochondrial permeability transition.

Proceedings of the National Academy of Sciences of the United States of America - 3 Mar 2026

Valinsky William C, Ray Robert P, Schaefer Kathy S, Grimm Jonathan B, Nicolini Carla, Lavis Luke D, Clapham David E

Abstract excerpt

The mitochondrial permeability transition (mPT) is an evolutionarily conserved destructive process that permeabilizes the inner mitochondrial membrane in response to calcium overload. The molecular mechanism underlying the mPT is not established. To unambiguously identify essential proteins, we designed two phenotypic assays for mitochondrial calcium overload and applied them to FACS-based CRISPR screening in...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 41739553
DOI: 10.1073/pnas.2535298123
PMCID: PMC12956895

Genes

BPTF
NF2
NLRX1
REST
MCU

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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