Article
Mechanistic insights into melanin-induced PCR inhibition and its NanoPCR-based mitigation.
Scientific reports - 22 Jan 2026
Vajpayee Kamayani, Srivastava Shriyansh, Sharma Shivkant, Gupta Swadha, Srivastava Ashutosh, Paida Vidhi, Dash Hirak Ranjan, Pappachan Anju, Shukla Ritesh K
Abstract excerpt
Melanin is a potent inhibitor of PCR, which interferes with forensic DNA typing by binding to Taq polymerase and disrupting its function, leading to allele dropout and decreased peak heights. Traditional mitigation measures, such as dilution and replacement of the polymerase, often result in the loss of DNA and are not suitable for low-template forensic samples. In this study, melanin-induced inhibition was...
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