Mechanistic Insights into Melanin-Induced PCR Inhibition and Its NanoPCR-Based Mitigation

Abstract excerpt

<title>Abstract</title> <p>Melanin is a potent inhibitor of PCR that inhibits forensic DNA typing by binding Taq polymerase and interfering with its function, resulting in allele dropout and decreased peak heights. Traditional mitigation measures, such as dilution or replacement of the polymerase, tend to result in loss of DNA and are not appropriate for low-template forensic samples. In this study, melanin-induc...

Topics

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• DNA
• DNA Fingerprinting
• Melanins
• Metal Nanoparticles
• Microsatellite Repeats
• Molecular Docking Simulation
• Molecular Dynamics Simulation
• Taq Polymerase

Identifiers and source

Literature Corpus work

ce888b7b-31f1-5333-b879-1f375cd01374

DOI

10.21203/rs.3.rs-7137528/v1