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Melanin-free DNA extraction: a rapid and high-efficiency protocol for molecular studies.

Molecular biology reports - 13 Jan 2026

El Bardai Sanae, El Agy Fatima, Tahiri Elousrouti Layla, Bouguenouch Laila, Ouldim Karim, Hammas Nawal, Chbani Laila

Abstract excerpt

BACKGROUND: A crucial step in molecular oncology diagnostics is the acquisition of high-quality genomic DNA. However, DNA extracted from melanocytes frequently contains melanin a potent PCR inhibitor that co-purifies with nucleic acids. This contamination can significantly impair both research applications and molecular diagnostic assays essential for targeted therapies. To address the inhibitory impact of...

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PMID: 41528658
DOI: 10.1007/s11033-025-11426-x

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