Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Comparative Methods to Improve the Detection of BRAF V600 Mutations in Highly Pigmented Melanoma Specimens.

PloS one - 1 Jan 2016

Frouin Eric, Maudelonde Thierry, Senal Romain, Larrieux Marion, Costes Valérie, Godreuil Sylvain, Vendrell Julie A, Solassol Jérôme

Abstract excerpt

Genotyping BRAF in melanoma samples is often challenging. The presence of melanin greatly interferes with thermostable DNA polymerases and/or nucleic acids in traditional polymerase chain reaction (PCR)-based methods. In the present work, we evaluated three easy-to-use strategies to improve the detection of pigmented DNA refractory to PCR amplification. These pre-PCR processing methods include the addition of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Aged
Share to Cell Line, Tumor

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Melanoma

Share to Mutation

Share to Polymerase Chain Reaction

Share to Proto-Oncogene Proteins B-raf

Share to Skin Neoplasms

Share to Skin Pigmentation

Source

Open in PubMed Open full text

Identifiers

PMID: 27466810
DOI: 10.1371/journal.pone.0158698
PMCID: PMC4965116

Genes

BRAF

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Comparative Methods to Improve the Detection of BRAF V600 Mutations in Highly Pigmented Melanoma Specimens.PMID 27466810Read ↗

Rapid BRAF Mutation Testing in Pigmented Melanomas.Semantic proximity Read ↗

Melanin-free DNA extraction: a rapid and high-efficiency protocol for molecular studies.Semantic proximity Read ↗

Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients.Semantic proximity Read ↗

Simple, Sensitive and Accurate Multiplex Detection of Clinically Important Melanoma DNA Mutations in Circulating Tumour DNA with SERS Nanotags.Semantic proximity Read ↗

Implementation of a companion diagnostic in the clinical laboratory: the BRAF example in melanoma.Semantic proximity Read ↗

Mutation Profile of B-Raf Gene Analyzed by fully Automated System and Clinical Features in Japanese Melanoma Patients.Semantic proximity Read ↗

Molecular biology methods to improve diagnosis and prognosis of melanocytic tumors.Semantic proximity Read ↗

A Sensitive Peptide Nucleic Acid Probe Assay for Detection of BRAF V600 Mutations in Melanoma.Semantic proximity Read ↗

Multi-center evaluation of the novel fully-automated PCR-based Idylla™ BRAF Mutation Test on formalin-fixed paraffin-embedded tissue of malignant melanoma.Semantic proximity Read ↗

Next-Generation Genotyping by Digital PCR to Detect and Quantify the BRAF V600E Mutation in Melanoma Biopsies.Semantic proximity Read ↗

Comparison of Five Different Assays for the Detection of BRAF Mutations in Formalin-Fixed Paraffin Embedded Tissues of Patients with Metastatic Melanoma.Semantic proximity Read ↗

Pre-analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.