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Article

Structure, Function and Dynamics of mCoral, a pH-Responsive Engineered Variant of the mCherry Fluorescent Protein with Improved Hydrogen Peroxide Tolerance.

International journal of molecular sciences - 23 Dec 2025

Zitti Athena, Aksakal Ozan, Vitsupakorn Danoo, Rizkallah Pierre J, Mikolajek Halina, Platts James A, Menzies Georgina E, Jones D Dafydd

Abstract excerpt

The red fluorescent protein mCherry is one of the most widely used fluorescent proteins in biology. Here, we have changed the chromophore chemistry by converting the thioether group of M66 to a thiol group through mutation to cysteine. The new variant, termed mCoral (due to its orange fluorescence hue), has similar brightness to mCherry but improved resistance to hydrogen peroxide. The variant is also responsive...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41516040
DOI: 10.3390/ijms27010154
PMCID: PMC12785414

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