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Article

pH-dependent transient conformational states control optical properties in cyan fluorescent protein.

Journal of the American Chemical Society - 4 Mar 2015

Laricheva Elena N, Goh Garrett B, Dickson Alex, Brooks Charles L

Abstract excerpt

A recently engineered mutant of cyan fluorescent protein (WasCFP) that exhibits pH-dependent absorption suggests that its tryptophan-based chromophore switches between neutral (protonated) and charged (deprotonated) states depending on external pH. At pH 8.1, the latter gives rise to green fluorescence as opposed to the cyan color of emission that is characteristic for the neutral form at low pH. Given the high...

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PMID: 25647152
DOI: 10.1021/ja509233r
PMCID: PMC4394632

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