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Article

Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity.

The Journal of biological chemistry - 27 Dec 2002

Rekas Agata, Alattia Jean-René, Nagai Takeharu, Miyawaki Atsushi, Ikura Mitsuhiko

Abstract excerpt

Yellow emission variants of green fluorescent protein (GFP) have been found useful in a variety of applications in biological systems due to their red-shifted emission spectrum and sensitivity to environmental parameters, such as pH and ionic strength. However, slow maturation properties and new requirements for more intense fluorescence necessitated further mutagenesis studies of these proteins. Venus, a new...

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Amino Acid Sequence
Bacterial Proteins
Crystallography, X-Ray
Luminescent Proteins
Models, Molecular

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Conformation

Recombinant Proteins

Sensitivity and Specificity

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PMID: 12370172
DOI: 10.1074/jbc.M209524200

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