Article
The crystal structure of the Y66L variant of green fluorescent protein supports a cyclization-oxidation-dehydration mechanism for chromophore maturation.
Biochemistry - 20 Apr 2004
Rosenow Matthew A, Huffman Holly A, Phail Marlene E, Wachter Rebekka M
Abstract excerpt
The crystal structure of a colorless variant of green fluorescent protein (GFP) containing the Y66L substitution has been determined to 1.5 A. Crystallographic evidence is presented for the formation of a trapped intermediate on the pathway of chromophore maturation, where the peptide backbone of residues 65-67 has condensed to form a five-membered heterocyclic ring. The hydroxyl leaving group remains attached to...
Topics
Join the communities discussing this publication.