Article
Enhanced specificity mutations perturb allosteric signaling in CRISPR-Cas9.
eLife - 15 Dec 2021
Nierzwicki Lukasz, East Kyle W, Morzan Uriel N, Arantes Pablo R, Batista Victor S, Lisi George P, Palermo Giulia
Abstract excerpt
CRISPR-Cas9 (clustered regularly interspaced short palindromic repeat and associated Cas9 protein) is a molecular tool with transformative genome editing capabilities. At the molecular level, an intricate allosteric signaling is critical for DNA cleavage, but its role in the specificity enhancement of the Cas9 endonuclease is poorly understood. Here, multi-microsecond molecular dynamics is combined with solution...
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