C-spine mutations of protein kinase C and Akt as a novel generalizable approach to create stable pseudokinases.

Abstract excerpt

Protein kinases function not only through their catalytic phospho-transfer activity but also by noncatalytic scaffold mechanisms. Introduction of mutations to inactivate catalysis provides a tool to differentiate between the two; however, kinase-inactivating mutations may alter the structure of the kinase domain and perturb scaffold functions. Here, we developed a strategy that prevents ATP binding, thereby...

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