Impeding pathways of intrinsic resistance in Escherichia coli confers antibiotic sensitization and resistance proofing.

Abstract excerpt

Pathways of intrinsic resistance in bacteria are promising targets for novel antibiotics and resistance breakers. Here, we used a genome-wide screen to identify single gene knockouts of Escherichia coli that were hypersusceptible to trimethoprim and chloramphenicol, two chemically diverse broad-spectrum antibiotics. Among the hits from our screen, knockouts of acrB, an efflux pump, and rfaG or lpxM, both involved...

Topics

• Escherichia coli
• Anti-Bacterial Agents
• Escherichia coli Proteins
• Trimethoprim
• Chloramphenicol
• Microbial Sensitivity Tests