Article
A base editor facilitates simultaneous purine and pyrimidine substitutions for ex vivo and in vivo mutagenesis screens.
Cell chemical biology - 18 Sept 2025
He Zhiyong, Xie Yuyang, Huang Honglin, Zhang Zhaoyu, Feng Shenjiong, Xu Runda, Chen Xuancheng, Gao Fei, Li Pan, Zhu Ming, Wu Sen, Du Xuguang
Abstract excerpt
Genetic mutations are closely linked to human diseases, yet the relationship between many mutations and their corresponding phenotypes remains poorly understood. Furthermore, tools to study the connection between nucleotide variations and phenotypes are limited. To address this issue, we developed ACGBEmax by fusing the dual-functional deaminase, engineered N-methylpurine DNA glycosylase, and evolved SOS response...
Read the complete abstract on PubMedTopics
- Animals
- Mice
- Purines
- Humans
- Mutagenesis
- Pyrimidines