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Article

Accurate and reproducible whole-genome genotyping for bacterial genomic surveillance with Nanopore sequencing data.

Journal of clinical microbiology - 9 Jul 2025

Prior K, Becker K, Brandt C, Cabal Rosel A, Dabernig-Heinz J, Kohler C, Lohde M, Ruppitsch W, Schuler F, Wagner G E, Mellmann A

Abstract excerpt

Despite recent advances in error rate reduction, until recently, Oxford Nanopore Technologies (ONT) sequences lacked the accuracy required for fine-scale bacterial genomic analysis. Here, recent software improvements of ONT and the ONT-core-genome multilocus sequence typing (cgMLST)-Polisher within the SeqSphere+ software were evaluated. We used short-read (Illumina) and long-read ONT sequences of 80...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 40511924
DOI: 10.1128/jcm.00369-25
PMCID: PMC12239720

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