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Evaluation of Oxford Nanopore Technologies workflows for genomic epidemiology of outbreak-associated bacterial isolates in the clinical setting.

Microbial genomics - 1 Feb 2026

Neuenschwander Stefan, Borcard Loïc, Gempeler Sonja, Terrazos Miani Miguel A, Casanova Carlo, Ramette Alban

Abstract excerpt

Accurate and efficient whole-genome sequencing (WGS) is crucial for clinical diagnostics and surveillance of bacterial infections. Here, we investigate the potential of a new Oxford Nanopore Technologies (ONT) workflow for WGS of clinically relevant bacterial isolates. Specifically, we assess the performance of R10.4.1 flow cells in combination with the V14 version of the transposase-based (RBK) library...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41665488
DOI: 10.1099/mgen.0.001626
PMCID: PMC12890258

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