Optimized De novo assembly of Haemophilus influenzae using oxford nanopore sequencing without short-read data on a graphical user interface-based galaxy platform: Accurate detection of MLST, PBP3 mutations, and phylogenetic clustering.

Abstract excerpt

Oxford Nanopore sequencing enables rapid, long-read, and cost-effective bacterial genome analysis; however, historically high error rates and reliance on command-line interface (CLI)-based workflows have limited its accessibility in clinical laboratories. We optimized a fully graphical user interface (GUI)-based workflow using the open-source Galaxy platform (usegalaxy.eu) for de novo assembly of Haemophilus...

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