How to recover from a bad start: adaptation of HIV-1 transcription start site mutants during serial passaging in culture.

doi:10.1128/jvi.00159-25

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

How to recover from a bad start: adaptation of HIV-1 transcription start site mutants during serial passaging in culture.

Journal of virology - 17 Jun 2025

Nikolaitchik Olga A, Chameettachal Akhil, Islam Saiful, Cheng Zetao, Delviks-Frankenberry Krista, Keele Brandon F, Pathak Vinay K, Hu Wei-Shau

Abstract excerpt

HIV-1 uses neighboring sequences as transcription start sites and generates multiple unspliced transcripts, including two major transcripts with three guanosines (3G) or one guanosine (1G) at the 5' end. Although only differing by 2-nt, 3G RNA and 1G RNA are functionally distinct. We have previously generated two mutants: the TTG virus mainly expresses 1G RNA, whereas the plusAC virus predominantly expresses 3G...

Topics

HIV-1
Humans
Virus Replication
RNA, Viral
Transcription Initiation Site
Mutation
Serial Passage
Transcription, Genetic
Reverse Transcription

Source

Open in PubMed Open full text

Identifiers

PMID: 40331822
DOI: 10.1128/jvi.00159-25
PMCID: PMC12172438

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics