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Article

Supersaturation mutagenesis reveals adaptive rewiring of essential genes among malaria parasites.

Science (New York, N.Y.) - 7 Feb 2025

Oberstaller Jenna, Xu Shulin, Naskar Deboki, Zhang Min, Wang Chengqi, Gibbons Justin, Pires Camilla Valente, Mayho Matthew, Otto Thomas D, Rayner Julian C, Adams John H

Abstract excerpt

Malaria parasites are highly divergent from model eukaryotes. Large-scale genome engineering methods effective in model organisms are frequently inapplicable, and systematic studies of gene function are few. We generated more than 175,000 transposon insertions in the Plasmodium knowlesi genome, averaging an insertion every 138 base pairs, and used this "supersaturation" mutagenesis to score essentiality for 98%...

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Share to Genes, Essential
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 39913589
DOI: 10.1126/science.adq7347
PMCID: PMC12131478

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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