Article
Quantitative insertion-site sequencing (QIseq) for high throughput phenotyping of transposon mutants.
Genome research - 1 Jul 2016
Bronner Iraad F, Otto Thomas D, Zhang Min, Udenze Kenneth, Wang Chengqi, Quail Michael A, Jiang Rays H Y, Adams John H, Rayner Julian C
Abstract excerpt
Genetic screening using random transposon insertions has been a powerful tool for uncovering biology in prokaryotes, where whole-genome saturating screens have been performed in multiple organisms. In eukaryotes, such screens have proven more problematic, in part because of the lack of a sensitive and robust system for identifying transposon insertion sites. We here describe quantitative insertion-site...
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