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Article

Evaluation of a next generation sequencing assay for Hepatitis B antiviral drug resistance on the oxford nanopore system.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Feb 2025

Payne Michael, Ritchie Gordon, Lawson Tanya, Young Matthew, Jang Willson, Stefanovic Aleksandra, Romney Marc G, Matic Nancy, Lowe Christopher F

Abstract excerpt

BACKGROUND: Next-generation sequencing (NGS) for Hepatitis B virus (HBV) antiviral resistance (AVR) testing is a highly sensitive diagnostic method, able to detect low-level mutant subpopulations. Our clinical virology laboratory previously transitioned from DNA hybridization (INNO-LiPA) to NGS, initially with the GS Junior System and subsequently the MiSeq. The Oxford Nanopore Technology (ONT) sequencing system...

Read the complete abstract on PubMed

Topics

Humans
Hepatitis B virus
High-Throughput Nucleotide Sequencing
Drug Resistance, Viral
Antiviral Agents

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Mutation

Genotype

Nanopores

Hepatitis B

DNA, Viral

Nanopore Sequencing

Source

Identifiers

PMID: 39818134
DOI: 10.1016/j.jcv.2025.105762

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