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Article

A Comparison of Sanger Sequencing and Amplicon-Based Next Generation Sequencing Approaches for the Detection of HIV-1 Drug Resistance Mutations.

Viruses - 14 Sept 2024

Biba Camilla, Fiaschi Lia, Varasi Ilenia, Paletti Chiara, Bartolini Niccolò, Zazzi Maurizio, Vicenti Ilaria, Saladini Francesco

Abstract excerpt

BACKGROUND: Next-generation sequencing (NGS) kits are needed to finalise the transition from Sanger sequencing to NGS in HIV-1 genotypic drug resistance testing. MATERIALS AND METHODS: We compared a homemade NGS amplicon-based protocol and the AD4SEQ HIV-1 Solution v2 (AD4SEQ) NGS kit from Arrow Diagnostics for identifying resistance-associated mutations (RAMs) above the 5% threshold in 28 plasma samples where...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 39339940
DOI: 10.3390/v16091465
PMCID: PMC11437444

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