Strategies to identify and edit improvements in synthetic genome segments episomally.

doi:10.1093/nar/gkad692

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Article

Strategies to identify and edit improvements in synthetic genome segments episomally.

Nucleic acids research - 13 Oct 2023

Rudolph Alexandra, Nyerges Akos, Chiappino-Pepe Anush, Landon Matthieu, Baas-Thomas Maximilien, Church George

Abstract excerpt

Genome engineering projects often utilize bacterial artificial chromosomes (BACs) to carry multi-kilobase DNA segments at low copy number. However, all stages of whole-genome engineering have the potential to impose mutations on the synthetic genome that can reduce or eliminate the fitness of the final strain. Here, we describe improvements to a multiplex automated genome engineering (MAGE) protocol to improve...

Topics

Escherichia coli
Genome, Bacterial
Chromosomes, Artificial, Bacterial
High-Throughput Nucleotide Sequencing
Plasmids
Mutation
Gene Editing
Genetic Engineering
Genes, Essential

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Identifiers

PMID: 37615546
DOI: 10.1093/nar/gkad692
PMCID: PMC10570025

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