Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Roadmap and Considerations for Genome Editing in a Non-Model Organism: Genetic Variations and Off-Target Profiling.

International journal of molecular sciences - 22 Nov 2024

Wattad Hanin, Molcho Jonathan, Manor Rivka, Weil Simy, Aflalo Eliahu D, Chalifa-Caspi Vered, Sagi Amir

Abstract excerpt

The CRISPR/Cas genome editing approach in non-model organisms poses challenges that remain to be resolved. Here, we demonstrated a generalized roadmap for a de novo genome annotation approach applied to the non-model organism Macrobrachium rosenbergii. We also addressed the typical genome editing challenges arising from genetic variations, such as a high frequency of single nucleotide polymorphisms, differences...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR-Cas Systems
Share to Gene Editing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genetic Variation

Share to High-Throughput Nucleotide Sequencing

Share to Molecular Sequence Annotation

Share to Polymorphism, Single Nucleotide

Share to Sex Chromosomes

Source

Open in PubMed Open full text

Identifiers

PMID: 39684244
DOI: 10.3390/ijms252312530
PMCID: PMC11641748

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Roadmap and Considerations for Genome Editing in a Non-Model Organism: Genetic Variations and Off-Target Profiling.PMID 39684244Read ↗

On genome editing in embryos and cells of the freshwater prawn Macrobrachium rosenbergiiSemantic proximity Read ↗

CRISPR and Long-Read Sequencing: A Perfect Match.Semantic proximity Read ↗

A catalog of gene editing sites and genetic variations in editing sites in model organisms.Semantic proximity Read ↗

The mosaicism of Cas-induced mutations and pleiotropic effects of scarlet gene in an emerging model system.Semantic proximity Read ↗

CRISPR-based dissection of miRNA binding sites using isogenic cell lines is hampered by pervasive noise.Semantic proximity Read ↗

DANGER ANALYSIS: RISK-AVERSE ON/OFF-TARGET ASSESSMENT FOR CRISPR EDITING WITHOUT A REFERENCE GENOMESemantic proximity Read ↗

Efficient CRISPR genome editing and integrative genomic analyses reveal the mosaicism of Cas-induced mutations and pleiotropic effects of <i>scarlet</i> gene in an emerging model systemSemantic proximity Read ↗

CRISPR/LbCas12a-mediated targeted mutation of Gracilariopsis lemaneiformis (Rhodophyta).Semantic proximity Read ↗

Evaluation of CRISPR gene-editing tools in zebrafishSemantic proximity Read ↗

Broad spectrum of CRISPR-induced edits in an embryonic lethal gene.Semantic proximity Read ↗

CRISPRing into the woods.Semantic proximity Read ↗

Deep Sequencing Reveals the Comprehensive CRISPR-Cas9 Editing Spectrum in Bombyx mori.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.