Article
Deep Sequencing Reveals the Comprehensive CRISPR-Cas9 Editing Spectrum in Bombyx mori.
The CRISPR journal - 1 Jun 2021
Ma Sanyuan, Wang Aoming, Chen Xiaoxu, Zhang Tong, Xing Weiqing, Xia Qingyou
Abstract excerpt
Application of the clustered regularly interspaced short palindromic repeats associated 9 (CRISPR-Cas9) technology has revolutionized biology by greatly enhancing the ability to introduce mutations into DNA for research and prospective therapeutic purposes. However, the understanding of Cas9 editing outcomes is still limited. Previously, it was considered that Cas9 introduces stochastic insertions or deletions...
Topics
- Animals
- Bombyx
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- DNA
- Female
- Gene Editing
- High-Throughput Nucleotide Sequencing
- Humans
- Mutation
- RNA, Guide, CRISPR-Cas Systems