Hook loop dynamics engineering transcended the barrier of activity-stability trade-off and boosted the thermostability of enzymes.

Abstract excerpt

The improvement of enzyme thermostability often accompanies the decreased activity due to the loss of the key regions' flexibility. As a representative structure, unlocking the potential of loop dynamics will not only provide new ideas for stabilization strategies, but also help to deepen the understanding of the relationship between enzyme structural dynamics and function. In this study, a creative "hook loop...

Topics

• Lipase
• Enzyme Stability
• Molecular Dynamics Simulation
• Protein Engineering
• Fungal Proteins
• Temperature
• Mutation
• Protein Conformation