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Article

Optimization of Mutation Sites in Bacillus Velezensis Serine Protease for Enhanced Acid and Thermal Stability in Animal Feed Applications.

Journal of agricultural and food chemistry - 8 Jul 2026

Huang Zhengxuan, Nassar Nourhan, Du Ni, Dong Minglin, Khan Samiullah, Khan Safir Ullah, Tan Jiajian, Khan Ibrar Muhammad, Wang Zaigui

Abstract excerpt

Serine proteases from Bacillus species are widely used in the feed industry, but their poor tolerance to high acidity and temperature limits their digestive efficacy in animals. To overcome this, we engineered the serine protease SprD1 from Bacillus velezensis D1 via structure-guided site-directed mutagenesis, using protein folding free energy (ΔΔG) analysis and loop flexibility modification. Multiple single and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 42334947
DOI: 10.1021/acs.jafc.5c16702

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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