Article
Multiplex-genome-editing based rapid directional improvement of complex traits in rice.
Plant biotechnology journal - 1 Sept 2024
Wei Yidong, Zhang Haomin, Fan Jiaxing, Cai Qiuhua, Zhang Zhixing, Wang Jinlan, Zhang Min, Yan Fengting, Jiang Jiahuan, Xie Hongguang, Luo Xi, Wei Linyan, Lin Yuelong, He Wei, Qu Mengyu, Zhang Xinxin, Zhu Yongsheng, Xie Huaan, Zhang Jianfu
Abstract excerpt
Although thousands of genes have been identified or cloned in rice (Oryza sativa) in the last two decades, the majority of them have only been separately characterized in specific varieties or single-gene modified backgrounds, thus limiting their practical application. We developed an optimized multiplex genome editing (MGE) toolbox that can efficiently assemble and stably express up to twelve sgRNA targets in a...
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