Cas9-PE: a robust multiplex gene editing tool for simultaneous precise editing and site-specific random mutation in rice.

Abstract excerpt

In molecular design breeding, the simultaneous introduction of desired functional genes through specific nucleotide modifications and the elimination of genes regulating undesired phenotypic traits or agronomic components require advanced gene editing tools. Due to limited editing efficiency, even with the use of highly precise editing tools, such as prime editing (PE), simultaneous editing of multiple mutation...

Topics

• CRISPR-Associated Protein 9
• Gene Editing
• Oryza
• Mutation
• Mutagenesis, Site-Directed
• Acetolactate Synthase
• Herbicide Resistance