Article
Construction of CRISPR/Cas9 Multiplex Genome Editing System in Rice.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Xiong Jie, Wang Chun, Wang Kejian
Abstract excerpt
Multiplex genome editing (MGE) technologies constitute essential tools for rapid genome modification of multiple targets in one gene or multiple genes simultaneously. However, the vector construction process is complicated, and the number of mutation targets is constrained using the conventional binary vectors. Here, we describe a simple CRISPR/Cas9 MGE system based on classical isocaudomer technique in rice,...
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