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Article

Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing.

Scientific reports - 19 Feb 2016

Liang Gang, Zhang Huimin, Lou Dengji, Yu Diqiu

Abstract excerpt

The CRISPR/Cas9-sgRNA system has been developed to mediate genome editing and become a powerful tool for biological research. Employing the CRISPR/Cas9-sgRNA system for genome editing and manipulation has accelerated research and expanded researchers' ability to generate genetic models. However, the method evaluating the efficiency of sgRNAs is lacking in plants. Based on the nucleotide compositions and secondary...

Topics

Base Sequence
CRISPR-Cas Systems
Gene Editing
Gene Order
Genetic Vectors
Genome, Plant
Genotype
Position-Specific Scoring Matrices
RNA, Guide, CRISPR-Cas Systems
RNA, Plant

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26891616
DOI: 10.1038/srep21451
PMCID: PMC4759811

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