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Multiplex CRISPR/Cas12a editing of ROC5, GS3, GW2, and LARGE2 reveals effects on yield-related traits in rice cultivar Kasalath.

Transgenic research - 21 Sept 2026

Janthabut Teerapong, Pongjaroenkit Saengtong, Khemkladngoen Naruemon, Lithanatudom Suparat K, Chanchay Pornchanan, Vuttipongchaikij Supachai, Sakulsingharoj Chotipa

Abstract excerpt

Sustainable improvement of rice yield requires coordinated modification of multiple agronomic traits, yet the agronomic outcomes of multiplex genome editing remain difficult to predict. Here, we applied CRISPR/Cas12a-mediated multiplex editing in the indica rice cultivar Kasalath to target six yield-related genes, Gn1a, TAD1, ROC5, GS3, GW2, and LARGE2, using a single Agrobacterium-delivered construct. Sanger...

Read the complete abstract on PubMed

Topics

Oryza
CRISPR-Cas Systems
Gene Editing
Plants, Genetically Modified
Plant Proteins
Phenotype

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 42766049
DOI: 10.1007/s11248-026-00511-x

Genes

LARGE2

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