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Article

A gradient phage-assisted continuous evolution method for screening suppressor tRNAs in Escherichia coli.

New biotechnology - 25 Sept 2024

Wang Fan, Liu Li-Hua, Wang Zhenyu, Jiang Ao, Wu Yi-Rui

Abstract excerpt

Suppressor tRNAs, notable for their capability of reading through the stop codon while maintaining normal peptide synthesis, are promising in treating diseases caused by premature termination codons (PTC). However, the lack of effective engineering methods for suppressor tRNAs has curtailed their application potential. Here, we introduce a directed evolution technology that employs phage-assisted continuous...

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PMID: 38777090
DOI: 10.1016/j.nbt.2024.05.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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